Science

Education:

Bachelor of Science, Biology – December 1999
Microbiology/Immunology Option; English Minor -Virginia Polytechnic Institute & State University

Work Experience:

Independent Contractor – August 2011-2012
Georgetown University School of Medicine

Educational coordinator and procurement manager for the Microbiology in Medicine course. Responsible for ordering and maintaining more than 125 products necessary for 9 educational labs and 200 students.

Research Assistant 3 – November 2007–December 2011
Georgetown University; Department of Microbiology and Immunology, Washington, D.C.

Cihlar Lab – Natural Product Drug Discovery Research in pharmacognosy:

Cranberry extract compounds – developed assays to find which inhibit planktonic growth and biofilm formation. Collaborated with the Ferreira Lab of The University of Mississippi – February 2008 Cranberry Extract Column chromatography separation, introduction to HPLC and NMR

School of Medicine – 2008-2010 – Educational coordinator and lab manager for the Microbiology in Medicine course. Duties included procurement, preparation and management of the class experiments, which included the maintenance of more than 25 strains including but not limited to Streptococcus, Neisseria, Staphylococcus and Escherichia.

Research Assistant 2 – June 2004-November 2007
Georgetown University, Department of Biology, Washington, D.C.

Elmendorf Lab – Investigation of attachment forces of Giardia lamblia. This biophysics study involved conducting flow cell protocol development, documentation of attachment behaviors and management of data from collaborating researchers’ publication of findings. This goal has largely been accomplished and is yielding data toward a manuscript and grant application. Refinement assay for detection of G-actin binding proteins in Giardia.

Responsibilities included maintenance of cultures, microscopy, image analysis, column chromatography, PAGE, protein expression, immunoblotting assays. Safety Officer in charge of radioactive material management as Radiation Safety Officer. Responsible for laboratory inventory and procurement and management of equipment maintenance schedules.

Biology of Parasitism Course – Marine Biological Laboratory (Woods Hole, Mass.) – June 2007
Teaching Assistant for Heidi Elmendorf’s Module: Understanding Transcriptional Regulation.

Responsibilities included project prep, procurement, lab setup, equipment maintenance and technical assistance, cDNA and 5′-RACE.

Research Assistant 2 – October 2000–March 2004
Georgetown University, Department of Microbiology and Immunology, Washington, D.C.

Cihlar Lab – Participated in drug discovery research with Candida albicans utilizing molecular-based technologies, including proteomics and genomics. The study involved: creation of protein constructs; expression of fusion proteins; protein purification methods, including column chromatography and dialysis; assay analysis; and protocol development. Investigated Candida albicans biofilm development. Laboratory safety as the Radiation Safety Officer.

Lab Technician 2 – February 2000-October 2000
MicroBioTest Inc., Sterling, Va.

Performed setup and implementation of antimicrobial efficacy tests, including Sporicidal, Minimum
Inhibitory Concentration (MIC), Use Dilution Test (UDT) and stress tests. Skilled in the use of
environmental swabs and wipes. Cell culture identification and maintenance, including the isolation of
duck primary hepatocytes.

Internship and Independent Research:
E. coli O157:H7 and Listeria monocytogenes Food Microbiology Department at American Meat Institute

Microscopy Experience:
Zeiss Axio Plan with Photometrics coolsnap camera and automated stage, Zeiss AxioPhot with Axiocam both with Improvision OpenLab and Volocity restoration and deconvolution ability, Nikon TE 2000 Inverted Microscope with DIC and epifluorescence optics, Uniblitz High Speed Shutter, Yokogawa CSU21 Spinning Disk Confocal Scan Head, AndorDV887-BI camera, Hamamatsu ORCAERCamera, ASIMS2000-xy motorized XY, Piezo systems objective or stage control, Bioptechs Live Cell Imaging Capabilities

Laboratory Skills:
Molecular techniques including: PCR and primer design; protein cloning; DNA purification; RTPCR; Agarose gel electrophoresis; acrylamide gel electrophoresis (PAGE); westernblots; protein expression and purification; Pierce BCA Protein Assays; columnchromatography; ELISA; media preparation; aseptic technique; experience in the isolation of pathogens; knowledge and understanding of current Good Laboratory Practices (cGLP); compliance to Standard Operating Procedures(SOP); and process validation. Certified in the handling of blood-borne pathogens and radioactive materials (RAM), as well as Biosafety Level 3 (BSL) practices

Laboratory Equipment:
Genios plate reader; Confocal Laser Scanning Microscope; Fluorescence Microscopy; Clone Manager; PCR thermocyclers; GST and other fusion protein expression systems; BCA and Pierce Protein assays; BioRad Mini-Protean 3 Gel System; API Microbial Identification Strips; custom fabrication of Pyrex equipment; and autoclave operation

Memberships:
American Society of Microbiologists

Computer Skills:
SyEd Clone Manager; Web Cutter; Internet applications; website development, maintenance, and php script implementation; Microsoft Office; Microsoft Excel; Adobe Photoshop